p16INK4a inactivation is not required to immortalize human mammary epithelial cells
نویسندگان
چکیده
منابع مشابه
Human papillomavirus type 16 E6-induced degradation of E6TP1 correlates with its ability to immortalize human mammary epithelial cells.
Recent analyses have identified a number of binding partners for E6, including E6AP, ERC55, paxillin, hDlg, p300, interferon regulatory factor 3, hMCM7, Bak, and E6TP1. Notably, association with E6 targets p53, E6TP1, myc, hMCM7, and Bak for degradation. However, the relative importance of the various E6 targets in cellular transformation remains unclear. E6 alone can dominantly immortalize nor...
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Inactivation of the ARF-p53 tumor suppressor pathway leads to immortalization of murine fibroblasts. The role of this pathway in immortalization of human epithelial cells is not clear. We analyzed the functionality of the p14(ARF)-p53 pathway in human mammary epithelial cells (MEC) immortalized by human papillomavirus 16 (HPV16) E6, the p53 degradation-defective E6 mutant Y54D, or hTERT. E6-MEC...
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Previous studies have demonstrated that normal mammary tissue contains a hierarchy of cell types that are ultimately and continuously derived from a self-sustaining mammary stem cell population. However, a suitable assay for detecting, quantifying and characterizing such cells in humans has been lacking. Here we show that histologically normal, bi-layered mammary epithelial structures containin...
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Abnormalities of chromosome number are frequently observed in cancers. The mechanisms regulating chromosome segregation in human cells are therefore of great interest. Recently it has been reported that human cells without an hSecurin gene lose chromosomes at a high frequency. Here we show that, after hSecurin knockout through homologous recombination, chromosome losses are only a short, transi...
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ژورنال
عنوان ژورنال: Oncogene
سال: 2002
ISSN: 0950-9232,1476-5594
DOI: 10.1038/sj.onc.1205902